Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Since everyone is talking about Tylenol let's actually talk about Tylenol. No, it doesn't cause autism But here's what it can do: Deplete glutathione: one of your body's most Tylenol's Impact on Glutathione and Liver Damage Researchers are studying a possible link between acetaminophen (Tylenol) use during pregnancy and higher autism or ADHD risk. It may lower glutathione, an important antioxidant for brain development. Glutathione: Master Antioxidant, Reducing Oxidative Stress, and Detoxification