Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Exposure to acetaminophen and all its metabolites upon 10, 15, and 20 mg kg intravenous acetaminophen in very preterm infants Pediatric Research Tylenol, Autism, And The Glutathione Connection OC Integrative Medicine Tylenol And Glutathione Invigor Medical Just say NO to Tylenol