Acetaminophen Anesthesia Key Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Myth busting, Acetaminophen version. "Avoid Acetaminophen (Tylenol) because it causes glutathione depletion". Truth?? As a pediatrician and ER doctor and as moms we're often asked about this. And we understand why: Interaction with Ethanol Alcohol Metabolism Exposure to acetaminophen and all its metabolites upon 10, 15, and 20 mg kg intravenous acetaminophen in very preterm infants Pediatric Research