Frontiers Acetaminophen induced liver injury: Molecular mechanism and treatments from natural products Tylenol and glutathione, actually explainedhere's the bullet points: * Lots of concern raised on social media re: glutathione depletion and acetaminophen use * Glutathione is used in the metabolism Is Tylenol Safe During Pregnancy? Understanding Risk Factors Seeking Health Glutathione: The "Master" Antioxidant By Bogdan Popa, M.D. Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.