Can Tylenol (acetaminophen) deplete glutathione levels in the body? The multiple mechanisms and modes of cell death after acetaminophen overdose Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Illustration of the effects of acetaminophen on glutathione, Download Scientific Diagram Frontiers Acetaminophen induced liver injury: Molecular mechanism and treatments from natural products