Acetaminophen Toxicity: How Genetic Susceptibility Combines with Environmental Factors Silymarin prevents acetaminophen induced hepatotoxicity via up regulation of the glutathione conjugation capacity in mice ScienceDirect Acetaminophen Pathway (therapeutic doses), Pharmacokinetics Illustration of the effects of acetaminophen on glutathione, Download Scientific Diagram Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.